|
KMID : 0380420100340040285
|
|
Journal of Prventive Veterinary Medicine
2010 Volume.34 No. 4 p.285 ~ p.291
|
|
|
Antibacterial effect of Saururus chinensis baill ethanol extract against Salmonella typhimurium infection in RAW 264.7 macrophage
|
|
Lee Jin-Ju
Kim Dong-Hyeok
Lim Jeong-Ju
Kim Dae-Geun
Kim Gon-Sub
Min Won-Gi
Lee Hu-Jang
Chang Hong-Hee
Kim Suk
|
|
|
Abstract
|
|
|
|
Salmonellosis is the commonest zoonosis worldwide that generally causes enterocolitis and foodborne poisoning which represents a considerable public health burden. Salmonella spp. are potential enteric pathogens and intracellularly replicates in host cells resulting in chronic infections. The medical treatments for salmonellosis have been difficult yet and had a serious problem including the increasing emergence of antibiotic resistance. The present report was designated to investigate the antibacterial effects of Saururus chinensis Baill ethanol extract (SCEE) on pure culture and infection with Salmonella enterica serovar Typhimurium (S. typhimurium) in murine derived macrophage RAW 264.7 cells. In determination of antibacterial activity of SCEE against S. typhimurium, bacterial viability was markedly decreased compared to the control. Also, SCEE significantly induced morphological change (p<0.05) of RAW 264.7 cells. In infection assay of S. typhimurium in RAW 264.7 cells pretreated with 100㎍/㎖ of SCEE, which is a non-cytotoxic concentration, bacterial uptake ability of macrophage was increased corresponding with morphological change, whereas bacterial survival rates within macrophage were markedly reduced compared with untreated control. Furthermore, nitric oxide (NO) production in SCEE-treated cells was slightly increased until 2 h but showed a tendency of decrease after 4 h until 24 h post infection compared with untreated control with S. typhimurium infection. Taken together, these findings demonstrated that SCEE has the antibacterial activity for S. typhimurium and the protective effects against S. typhimurium infection through activating murine macrophage independent on NO, suggesting that SCEE may be beneficial on the disease caused by intracellularly replicating pathogens as a safe alternatives of conventional chemotherapies.
|
|
|
KEYWORD
|
|
|
Saururus chinensis Baill, Ethanol Extract, Antibacterial Effect, Salmonellosis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|